Fig. 5

Angiogenic activity of PlaMSC-exo in vivo. a Laser Doppler blood flow analysis. Superficial blood flow on auricles significantly increased on days 3 and 6 following infusion of PlaMSC-exo (4.4 μg total protein, closed circles) compared to control (open circles). Six mice were used for the analyses. Mean differences in blood flow (Flux-PU) between day 0 and day 3 or 6 were compared between PlaMSC-exo and controls. A line with the same style denotes the same animal. Means indicated by horizontal lines. *P < 0.05, **P < 0.01, determined by Student’s t tests. b Representative histological sections of murine auricles. Murine auricles were excised 3 days after infusion of PlaMSC-exo and the frozen sections were stained with HE for histological examination. Infusion of PlaMSC-exo (4.4 μg in total) resulted in increased small blood vessels as indicated by the arrows. PBS (–) was infused as negative control. Scale bar = 300 μm. Right panels show enlarged images of square regions in left panels. Scale bar = 100 μm. PlaMSC-exo exosomes derived from MSCs isolated from human term placental tissue