Fig. 1
From: Long-term safety of human retinal progenitor cell transplantation in retinitis pigmentosa patients
Characterization of retinal progenitor cells (RPCs). a Undifferentiated RPCs were stained with early eye field markers, including paired box protein 6 (PAX6) and SRY (sex determining region Y)-box 2 (SOX2), and for the immature neural cell marker Nestin. RPCs were stained negative for glial fibrillary acidic protein (GFAP). b Flow cytometry profiles of RPCs for subpopulations expressing PAX6, SOX2, Nestin, and GFAP, respectively. c The graph shows gene expression of retinal progenitor and mature retinal markers (fold increase) in hRPCs compared with human embryonic stem cells (hESCs) assayed by real-time quantitative polymerase chain reaction (RT-qPCR). d Normal female (46 XX) karyotype of the clinical RPCs. e Photoreceptor differentiation by retinoic acid (RA) treatment in vitro. RA(+) groups showed RPCs were positive for the mature photoreceptor markers recoverin and rhodopsin and negative for Ki67 after RA inducing. RA(-) groups are the controls