Fig. 5

MSC-MVs decreased endothelial cell apoptosis and improved cell viability via HGF. Endothelial cell apoptosis was assessed using TUNEL assay kit. As a and b showed, the apoptosis index of endothelial cells were increased after LPS challenge, while treatment with MSC-CM or MSC-MVs treatment significantly reduced the apoptosis index of endothelial cells. Once again, the effect of MSC-MVs was significantly restrained after HGF gene knockdown. MTT assay confirmed that treatment with MSC-MVs restored cell viability to a greater extent than LPS stimulation alone. However, the effect of MSC-MVs was significantly inhibited after HGF gene knockdown (c). (n = 3, *p < 0.05, **p < 0.01 vs. control group; ^# p < 0.05 vs. LPS group; &p < 0.05 &&p < 0.01 vs. MSC-MVs group). HGF hepatocyte growth factor, LPS lipopolysaccharide, MSC-CM mesenchymal stem cells conditioned medium, MSC-MVs mesenchymal stem cells microvesicles, MSCs, mesenchymal stem cells