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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Local application of IGFBP5 protein enhanced periodontal tissue regeneration via increasing the migration, cell proliferation and osteo/dentinogenic differentiation of mesenchymal stem cells in an inflammatory niche

Fig. 1

Depletion of IGFBP5 inhibited the functions of PDLSCs in inflammatory condition. TNFα (10 ng/ml) was used to treat PDLSCs. a Real-time PCR results. GAPDH was used as an internal control. Short hairpin RNAs were utilized to infect PDLSCs and to silence IGFBP5 (IGFBP5sh) or scramble shRNA (Scramsh). b, c The scratch-simulated wound migration assay results. Scale bar: 100 μm. d, e The Transwell chemotaxis assay results. Scale bar: 50 μm. f ALP activity. g Alizarin red staining. h Cell Counting Kit-8 assay results. Student’s t test was implemented to test statistical significance. Error bars represent SD (n = 3). *P ≤ 0.05; **P ≤ 0.01. IGFBP5 insulin-like growth factor binding protein 5

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Stem Cell Research & Therapy

ISSN: 1757-6512