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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Local application of IGFBP5 protein enhanced periodontal tissue regeneration via increasing the migration, cell proliferation and osteo/dentinogenic differentiation of mesenchymal stem cells in an inflammatory niche

Fig. 6

BCOR negatively regulated IGFBP5 expression by association with KDM6B in PDLSCs. a Short hairpin RNAs were used to infect PDLSCs and silence BCOR (BCORsh) or scramble shRNA (Scramsh). The knockdown of BCOR in PDLSCs was determined by real-time RT-PCR. GAPDH was utilized as an internal control. b The knockdown of BCOR promoted IGFBP5 expression in PDLSCs as verified by real-time RT-PCR. c Western blot showed the overexpression of BCOR in PDLSCs. d Real-time PCR results showed that overexpression of BCOR decreased IGFBP5 expression in PDLSCs. e Co-IP assay results showed the formation of BCOR-KDM6B protein complex in PDLSCs. β-actin was used as an internal control. f ChIP assay demonstrated that overexpression of BCOR resulted in changes in histone K27 trimethylation (H3K27me3) in the promoter of IGFBP5. Student’s t test was utilized to test statistical significance. Error bars represent SD (n = 3). *p ≤ 0.05; **p ≤ 0.01. BCOR BCL6 co-repressor, GAPDH glyceraldehyde-3-phosphate dehydrogenase, IGFBP5 insulin-like growth factor binding protein 5, KDM6B lysine (K)-specific demethylase 6B

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