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Table 1 Number of ESC-like and AP+Nanog+ colonies obtained on OSKM/mir-524 co-transduction of HFF-1 cells

From: miR-524-5p of the primate-specific C19MC miRNA cluster targets TP53IPN1- and EMT-associated genes to regulate cellular reprogramming

Transduction

Colonies in experiment (n)

Total colonies (n)

SD

Reprogramming efficiency (%)

1

2

3

OSKM only

4

3

6

12

1.53

0.005

OSKM + CD511

6

4

3

12

1.53

0.005

OSKM + mir-524

8

7

12

27*

2.65

0.012*

  1. ESC-like and AP+Nanog+ colonies in triplicate wells in a 12-well plate were counted under a microscope as described in Methods. Reprogramming efficiency was calculated as the total number of AP+Nanog+ colonies generated from the total number of transduced HFF-1 cells in three independent experiments. *p < 0.05 compared with OSKM only. AP alkaline phosphatase, ESC embryonic stem cell, SD standard deviation
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Stem Cell Research & Therapy

ISSN: 1757-6512