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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Human umbilical cord-derived mesenchymal stem cells ameliorate insulin resistance by suppressing NLRP3 inflammasome-mediated inflammation in type 2 diabetes rats

Fig. 1

PA–LPS stimulated inflammatory cytokine release in insulin-resistant HepG2 cells. HepG2 cells were incubated with BSA, PA-loaded BSA, LPS, and PA–LPS and then stimulated with insulin (100 nm) for 3 h. (a) Glycogen content in cells determined using a glycogen assay kit. (b) A quantity of 300 μl media in all groups was measured before and after the inducer by glucose assay kit, whose difference values were further normalized with cellular protein concentration. (c–e) Levels of IL-1β, IL-18, and TNF-α quantified by a commercial ELISA kit. *P < 0.05, **P < 0.01, ***P < 0.001. BSA bovine serum albumin, IL interleukin, LPS lipopolysaccharide, PA palmitic acid, TNF tumor necrosis factor

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