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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Human umbilical cord-derived mesenchymal stem cells ameliorate insulin resistance by suppressing NLRP3 inflammasome-mediated inflammation in type 2 diabetes rats

Fig. 3

Effect of LY294002 on PI3K signaling and inflammation. (a–d) Quantitative RT-PCR analysis of gene expression in HepG2 cells from blank, PA + LPS, LY294002, and UC-MSC groups. (e–g) Levels of cytokines in cell-free culture supernatants assayed by ELISA. (h) Fold change of caspase-1 activity in six groups. (i) Immunoblotting analysis of NLRP3, p-IRS (Ser307), IRS, PI3K, p-AKT (Thr308), AKT, and Glut4 levels in HepG2 cells performed in the presence of 100 nm insulin. *P < 0.05, **P < 0.01. Images are representative of four independent experiments. IL interleukin, LPS lipopolysaccharide, PA palmitic acid, TNF tumor necrosis factor, UC-MSC umbilical cord-derived mesenchymal stem cell

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