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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Microbiota regulates bone marrow mesenchymal stem cell lineage differentiation and immunomodulation

Fig. 1

Characterization and proliferation of GF, SPF, and ConvD BMMSCs. a Flow cytometry analysis shows that BMMSCs derived from SPF and GF mice had similar positive markers (CD73, CD90, CD105, Sca1, and CD166) and negative markers (CD34 and CD45). b Colony forming unit experiments show BMMSCs derived from GF mice had higher colony forming rate compared to that from SPF and ConvD mice (21.67 ± 0.88 vs 14.67 ± 1.20 and 15.33 ± 1.2). c CCK8 analysis shows BMMSCs from GF mice had higher proliferation capacity compared to that from SPF and ConvD mice. d PCR analysis shows that no bacterium was detected in GF feces using universal bacteria primers 515F and 806R. All experimental data verified in at least three independent experiments. Error bars represent the SEM from the mean values. **P < 0.001; *P < 0.05. ConvD conventionalized, GF germ free, SPF specific pathogen free, N.S. not significant

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