Fig. 4
From: Microbiota regulates bone marrow mesenchymal stem cell lineage differentiation and immunomodulation
Single-cell RNA-sequencing identifies differences of three major pathway categories between GF and SPF/ConvD BMMSCs. a Unsupervised cell population clustering shows SPF and ConvD BMMSCs clustered together, while GF BMMSCs distributed into two parts. b Correlation analysis shows most mRNA expression in SPF and ConvD BMMSCs was related, distinct from GF BMMSCs. c Pathway analysis shows the different expressing genes between GF and SPF/ConvD BMMSCs. d Different gene-related protein interaction networks show that chemokines such as CXCL1, CCL2, and IL10 play a central role in BMMSC immunomodulation. GF germ free, SPF specific pathogen free