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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Synergistic effects on mesenchymal stem cell-based cartilage regeneration by chondrogenic preconditioning and mechanical stimulation

Fig. 1

Schematic diagram of the study design. a Procedure for deriving manipulated mesenchymal stem cells (M-MSCs) via chondrogenic preconditioning. To obtain chondrogenic preconditioned M-MSCs, MSCs were firstly incubated with chondrogenic induction medium for 7 days which was then followed by basal growth medium for another 7 days. b UV-initiated crosslinking of methacrylated hyaluronic acid (MeHA) laden with cells (20 × 106 cells/mL). c Compressive loading to the cell-seeded MeHA hydrogel in a bioreactor. M-MSCs or untreated MSCs photoencapsulated in MeHA hydrogel were subjected to free loading or dynamic loading in a bioreactor for 14 days. d Subcutaneous implantation in nude mice. After 14 days of culture in a bioreactor, the constructs were subcutaneously implanted in nude mice for 30 days. e Implantation in osteochondral defect in rats. After 14 days of culture in a bioreactor, the constructs were implanted in an osteochondral defect in rats. Samples were harvested after 8 weeks

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