Fig. 1

a Schematic of Muse-AT cell generation from lipoaspirate material. Different cellular components are present in adipose tissue (i.e., adipocytes, endothelial cells (ECs), adipose stem cells (ASCs), adipose tissue macrophages, and Muse-AT cells). Adipose tissue (lipoaspirate material) first exposed to collagenase for 30 minutes at 37 °C, and then for 12 hours under severe cellular stress conditions (i.e., long-term collagenase incubation, lack of nutrients, low temperature, and hypoxia). Only a cluster of Muse-AT cells survived such stress. b Expression of pluripotent stem cell markers SSEA4, Oct-4, Sox-2, and TRA1-6 in Muse-AT cells. c Expression of CD markers in Muse cells indicating an immunophenotype. d Evidence of a normal karyotype in Muse cells. e Muse-AT cells do not form teratomas after 6-month implantation in testis (right) in comparison with control, sham-injected testis (left). Muse-AT adipose tissue-derived multilineage differentiating stress enduring (c Reproduced from Figure 2 in Gimeno et al. [22] under CC-BY license) (d Reproduced from Figure 4 in Gimeno et al. [22] under CC-BY license) (e Reproduced from Figure 4 in Gimeno et al. [22] under CC-BY license)