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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Omentin-1 effects on mesenchymal stem cells: proliferation, apoptosis, and angiogenesis in vitro

Fig. 3

PI3K/Akt was involved in the antiapoptotic effects of omentin-1 on MSCs under oxidative stress. MSCs were pretreated with omentin-1 (0–800 ng/ml) for 1 h and continuously incubated with 400 μM H2O2 for 6 h. In order to identify the role of the PI3K/Akt signaling pathway on the antiapoptotic actions of omentin-1, LY294002 (LY; 20 μm/L) was added to the MSC medium for 1 h prior to omentin-1 (800 ng/ml) treatment. a,c Cell apoptosis was confirmed by Annexin V/propidium iodide (PI) and b,d Hoechst 33342 staining. Data are presented as the mean ± SD of three separate experiments. $ P < 0.05, versus the control group; *P < 0.05, versus the H2O2 group; # P < 0.05, versus the 800 ng/ml omentin-1 treated group. Cont. control

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