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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Omentin-1 effects on mesenchymal stem cells: proliferation, apoptosis, and angiogenesis in vitro

Fig. 4

Effects of omentin-1 on ROS and the mitochondrial apoptosis pathway. MSCs were pretreated with omentin-1 (0–800 ng/ml) for 1 h and followed by exposure to H2O2 for 6 h. LY294002 (LY; 20 μm/L) was added into the MSC medium for 1 h prior to omentin-1 (800 ng/ml) treatment. a Intracellular ROS was assessed with DCFH-DA by flow cytometry. b The mitochondrial transmembrane potential of MSCs was analyzed by flow cytometry. c The expression of proteins associated with mitochondrial apoptosis pathways was evaluated by Western blotting. Data are presented as the mean ± SD of three separate experiments. $ P < 0.05, versus the control group; *P < 0.05, versus the H2O2 group; # P < 0.05, versus the 800 ng/ml omentin-1 treated group. Cl.caspase-3 cleaved caspase-3, Cont. control

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