Fig. 5

Effects of omentin-1 on the capillary-like tube formation capacity of the conditioned medium and angiogenic factor secretion. The media were conditioned by only mesenchymal stem cells (MSCs), omentin-1 (800 ng/ml) preincubated MSCs, or LY294002 (LY) and omentin-1 (800 ng/ml) preincubated MSCs. Then the conditioned media were collected. Basal medium was used as a negative control. a,b HUVECs were incubated in each conditioned medium for 6 h in Matrigel, and the total length of the tube network per field was quantified. c Concentrations of vascular endothelial growth factor (VEGF), fibroblast growth factor-2 (FGF-2), hepatocyte growth factor (HGF), and insulin-like growth factor-1 (IGF-1) in the conditioned medium were measured by ELISA and normalized to cell counts. d Concentrations of interleukin-1 receptor antagonist (IL-1ra), tumor necrosis factor-stimulated gene-6 (TSG-6), interleukin (IL)-6, and IL-8 in the conditioned medium were measured by ELISA and normalized to cell counts. Data are presented as the mean ± SD of three separate experiments. *P < 0.05, versus the basal medium; ^# P < 0.05, versus the conditioned medium from the untreated MSC group; ^$ P < 0.05, versus the conditioned medium from the omentin-1-treated group