Fig. 1

NSC culture and characterization. Morphological analysis of NSCs from mouse cortex. Phase-contrast photomicrographs of suspension neurospheres (a) and monolayer culture cells (b). Scale bar = 100 μm. Identification of cultured NSCs. Fluorescent photomicrographs of NSCs for Nestin (c), SOX2 (d), GFAP (e), and DCX (f). Nuclei were stained with DAPI. Scale bar = 100 μm. Quantifications for GFAP⁺, Nestin⁺, SOX2⁺, and DCX⁺ cells (g)