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Fig. 10 | Stem Cell Research & Therapy

Fig. 10

From: Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice

Fig. 10

Adjudin-pretreated NSCs lessened Evans blue and IgG extravasation and inhibited ZO-1 and occludin degradation. Photographs represent the perfused brains after EB injection (a). Quantification of extravasated EB dye. The dye was analyzed by a spectrophotometer at 610 nm (b). n =14 for PBS and untreated NSC groups, and n = 19 for adjudin-pretreated NSC group. Immunofluorescence staining for IgG (red) in sham, PBS, nonpretreated NSC, and adjudin-pretreated NSC groups at 3 days after cell transplantation, with DAPI staining for contrast (c). Scale bar =100 μm. Quantification of the IgG fluorescent intensity in each group (d). n = 8 in each group. Sections from ischemic penumbra were stained for ZO-1 (green) and occludin (green), and then costained with endothelial marker CD31 (red) (e). Nuclei were stained with DAPI. Scale bar = 100 μm. Representative western blot analysis for ZO-1 and occludin protein levels in the ischemic penumbra from sham, PBS, nonpretreated NSC, and adjudin-pretreated NSC groups at 3 days after cell transplantation (f). Quantification of densitometric values of the protein bands normalized to the respective β-tubulin and actin (g). n = 6 in each group. Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. NSC neural stem cell, PBS phosphate-buffered saline

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