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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice

Fig. 3

Adjudin-pretreated NSCs inhibited H2O2-induced oxidative stress. Bar graphs show mRNA levels of iNOS, catalase, and SOD2: relative mRNA expression of iNOS (a), catalase (b), and SOD2 (c) normalized to Rplp0. Adjudin-pretreated NSCs inhibited H2O2-induced oxidative stress at protein levels. Representative western blot analysis showed that adjudin inhibited H2O2-induced iNOS upregulation, and increased catalase and SOD2 protein levels in the presence of 0.1 mM H2O2 (d). Quantification of densitometric value of the protein bands normalized to the respective β-tubulin (eg). Bars represent mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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