Fig. 5

Adjudin-pretreated NSCs reduced brain infarct volume and improved neurobehavioral outcome after I/R. Representative sets of cresyl violet staining of brain sections from mice treated with PBS, untreated NSCs, and adjudin-pretreated NSCs 3 days following tMCAO. Dashed line shows the border of the infarct area (a). Quantification of infarct volumes (b). n = 8 in each group. Adjudin-pretreated NSCs significantly ameliorated neurological deficits 3 days after transplantation when compared to the PBS or NSC group. n = 14 for PBS and untreated NSC group, and n = 19 for adjudin-pretreated NSC group (c). Adjudin-pretreated NSCs promoted the phosphorylation of Akt in ipsilateral cortex (d) and striatum (e) after tMCAO. Representative western blot assay showing that adjudin increased the p-Akt protein level 3 days after tMCAO compared with sham, PBS, and NSC groups. Quantification of densitometric value of the protein bands of cortex and straitum normalized to total Akt (f, g). n = 6 in each group. Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. NSC neural stem cell, PBS phosphate-buffered saline