Fig. 6

Adjudin-pretreated NSCs inhibited cytokine production and activation of microglia after I/R. Relative mRNA expression of IL-6, IL-1β, and TNF-α normalized to Rplp0 detected 3 days following cell transplantation. Expression of IL-6 (a, d), IL-1β (b, e), and TNF-α (c, f) in ipsilateral cortex and striatum shown in the NSC and adjudin-pretreated NSC groups. n =6 in each group. Immunofluorescence staining for CD11b (green) in the sham group, and tMCAO groups with either PBS injection, NSC injection, or adjudin-pretreated NSC injection. Samples were acquired 3 days after cell transplantation, with DAPI staining for contrast (g). Scale bar =100 μm. Quantification of CD11b immunofluorescence intensity in each group (h). n = 8 in each group. Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. DAPI 4,6-diamidino-2-phenylindole, NSC neural stem cell, PBS phosphate-buffered saline