Fig. 4From: The effects of melatonin on colonization of neonate spermatogonial mouse stem cells in a three-dimensional soft agar culture systemImmunofluorescent staining of SSC colonies. SSCs were positive for Plzf in the nuclei and α6 integrin in the cytoplasm (green) (b, e), and nuclei were stained with DAPI (blue) (a, d). c, f A merge of Plzf and α6 integrin DAPI. A single SSC cell (arrowhead) proliferated and created a colony of SSCs (arrow). Scale bars = 50 μmBack to article page