Fig. 7From: The effects of melatonin on colonization of neonate spermatogonial mouse stem cells in a three-dimensional soft agar culture systemFlow cytometry analysis for the detection of reactive oxygen species (ROS) in different groups. a Fresh group, b control group (without melatonin), c experimental group (melatonin 100 μM). M2: DCFDA-negative cells, M1: DCFDA-positive cells. d ROS production of SSCs before and after SACS analyzed by flow cytometry. Note the significantly lower production of ROS after SACS with 100 μM melatonin compared to the control group. Data are expressed as means ± SD. ***P ≤ 0.0001Back to article page