Table 1 The primer sequences for ID4, Plzf, c-kit, and GAPDH genes
Gene name | Sequence | Product size (bp) | Annealing temperature (° C) |
|---|---|---|---|
ID4 | Forward: 5'- TCCCGCCCAACAAGAAAGTC -3' | 102 | 60.54 |
Reverse: 5'- TCAGCAAAGCAGGGTGAGTC-3' | |||
Plzf | Forward: 5'- CGTTGGGGGTCAGCTAGAAAG -3' | 301 | 57.14 |
Reverse: 5'- CACCATGATGACCACATCGC-3' | |||
c-kit | Forward: 5'- AACAACAAAGAGCAAATCCAGG -3' | 200 | 57.67 |
Reverse: 5'- GGAAGTTGCGTCGGGTCTAT -3' | |||
GAPDH | Forward: 5'-AGCAAGGACACTGAGCAAGAG-3' | 151 | 60.35 |
Reverse: 5'- TCGTTCCTCTGATCGTTTCC -3' |