Fig. 12

Invasive lung function test in 6-week-old and 10-week-old mice (n = 5–7). a–d There were no significant differences in the baseline of either Rrs at 6 weeks (a) and 10 weeks (c) or Crs at 6 weeks (b) and 10 weeks (d) between groups at both time points. e, f By week 6, compared to control group, Rrs was significantly increased (e) and Crs was significantly decreased (f) at the 100 mg/ml methacholine dose in the injured group, but this effect was diminished with early hAEC treatment and also improved slightly in the late treatment groups. g By week 10, Rrs was significantly increased at 100 mg/ml methacholine dose in the injured group compared to the control group; both treatment groups did not significantly affect Rrs. h Crs did not change significantly across all experimental groups. i, j PV loop at week 6 (i) and week 10 (j). There was a significant upward shift of the PV loop in the injured group compared to the control group at both time points. By week 6, while the PV loop of the early hAEC treatment group was intermediate to both control and saline-treated injured groups, late treatment had no effect on the position of the PV loop. However, the PV loop position was not altered by either early or late hAEC treatment in week 10. Data expressed as mean ± standard error of mean (SEM). Statistical significance determined with one-way ANOVA accompanied by the Bonferroni post-hoc test. *p < 0.05, **p < 0.01, ***p < 0.001. hAEC human amnion epithelial cell, LPS lipopolysaccharide, H hyperoxia