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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human amnion cells reverse acute and chronic pulmonary damage in experimental neonatal lung injury

Fig. 4

FACS in mouse lung cells on PND7 (n = 6–8). a FACS gating strategies for CD4+ T cells, CD8+ T cells, neutrophils (CD45+Ly6GhiCD11bhiCD11ChiF4/80) and natural killer cells (CD45+NKp46+CD3). b FACS gating strategies for CD103+CD11c+ dendritic cells (CD45+CD103hiCD11ChiLy6C-F4/80loMHCIIhiCD11bloSiglec-F cells) and interstitial macrophages (CD45+CD103loCD11CloLy6CloF4/80intMHCIIhiCD11bloSiglec-F). c, d There were no differences in CD4+ and CD8+ populations across groups. e There was no difference in the percentage of neutrophils between groups. fh Percentage of CD103+CD11c+ dendritic cells (f) and natural killer cells (g) increased in the injured group, but was only significantly returned to control levels by early treatment. h Percentage of interstitial macrophages significantly increased in the injured animals, but was brought back to control levels by early treatment. Data expressed as mean ± standard error of mean (SEM). Statistical significance determined with one-way ANOVA accompanied by the Bonferroni post-hoc test. **p < 0.01, ****p < 0.0001. FSC forward scatter, SSC side scatter, NK natural killer, DC dendritic cell, hAEC human amnion epithelial cell

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