Fig. 4

FACS in mouse lung cells on PND7 (n = 6–8). a FACS gating strategies for CD4⁺ T cells, CD8⁺ T cells, neutrophils (CD45⁺Ly6G^hiCD11b^hiCD11C^hiF4/80^–) and natural killer cells (CD45⁺NKp46⁺CD3^–). b FACS gating strategies for CD103⁺CD11c⁺ dendritic cells (CD45⁺CD103^hiCD11C^hiLy6C^-F4/80^loMHCII^hiCD11b^loSiglec-F^– cells) and interstitial macrophages (CD45⁺CD103^loCD11C^loLy6C^loF4/80^intMHCII^hiCD11b^loSiglec-F^–). c, d There were no differences in CD4⁺ and CD8⁺ populations across groups. e There was no difference in the percentage of neutrophils between groups. f–h Percentage of CD103⁺CD11c⁺ dendritic cells (f) and natural killer cells (g) increased in the injured group, but was only significantly returned to control levels by early treatment. h Percentage of interstitial macrophages significantly increased in the injured animals, but was brought back to control levels by early treatment. Data expressed as mean ± standard error of mean (SEM). Statistical significance determined with one-way ANOVA accompanied by the Bonferroni post-hoc test. **p < 0.01, ****p < 0.0001. FSC forward scatter, SSC side scatter, NK natural killer, DC dendritic cell, hAEC human amnion epithelial cell