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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Human amnion cells reverse acute and chronic pulmonary damage in experimental neonatal lung injury

Fig. 5

Changes in inflammatory cytokine levels measured from mouse lung on PND7 (n = 6–8). In our experience, the detection limits for the cytokines are: IL-1β, 10.36 pg/ml; TNF-α, 5.8 pg/ml; IL-2, 3.72 pg/ml; IL-4, 6.98 pg/ml; IL-6, 0.74 pg/ml; IL-10, 2.95 pg/ml; IFN-γ, 1.84 pg/ml; GM-CSF, 6.2 pg/ml; LIF, 1.75 pg/ml; MIP-2, 6.05 pg/ml; MCP-1, 22.4 pg/ml. IL-1β (a) and TNF-α (b) levels in the dosage study: both were increased in the injured group, but all dosages of hAECs significantly reduced IL-1β and TNF-α back to control levels. IL-1β (c) and TNF-α (d) levels in the time-of-dose effect study: both early and late hAEC administration significantly reduced IL-1β and TNF-α back to control levels. IL-2 (e) and IL-10 (f) levels in dosage groups: there were no differences in IL-2 and IL-10 levels between groups. Data expressed as mean ± standard error of mean (SEM). Statistical significance determined with one-way ANOVA accompanied by the Bonferroni post-hoc test. *p < 0.05, **p < 0.01, ***p < 0.001. hAEC human amnion epithelial cell, IL interleukin, TNF tumour necrosis factor

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