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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: TGFβ3 recruits endogenous mesenchymal stem cells to initiate bone regeneration

Fig. 4

Knockdown of Smad3 in vascular cells inhibited TGFβ3-induced hBMSC migration. a Expression of Smad3 in hUVECs and hUASMCs were transfected with siRNA Smad3 as assessed by western blot analysis. b Relative density of Smad3 for (a). c Secretion of MCP1 in different cells. d Transwell assay for hBMSC migration in the coculture system of hBMSC and vascular cells with or without knockdown of Smad3. Migrated cells were stained purple with crystal violet. Scale bar: 100 μm. **P < 0.01, ****P < 0.001. hUASMC human umbilical artery smooth muscle cell, hUVEC human umbilical vein endothelial cell, MSC mesenchymal stem cell, siRNA small interfering RNA, TGFβ3 transforming growth factor beta-3

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