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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human iPSC-derived chondrocytes mimic juvenile chondrocyte function for the dual advantage of increased proliferation and resistance to IL-1β

Fig. 4

hiChondrocytes mimic juvenile chondrocytes in generation of superior cartilage in PEG-CS hydrogels in vivo. (a) Confocal microscopy for representative immunofluorescence staining for chondrocyte markers Sox9, Col2a1, and Col10a1 in hydrogel-capsulated hiChondrocytes cultured for 4 weeks in vivo. Scale bar, 50 μm. (b) Quantification of GAG production in hiChondrocyte-derived, juvenile chondrocyte-derived, and adult chondrocyte-derived cartilage constructs in vivo. The GAG content of the control acellular hydrogel was 60 μg. (c) Representative immunofluorescence staining for aggrecan, Col2a1, and Col10a1 in hydrogels containing hiChondrocytes, juvenile chondrocytes, and adult chondrocytes that were transplanted in mice and harvested after 4 weeks. Scale bar, 100 μm. Data represent two independent donors for each chondrocyte type and three replicate experiments. GAG glycosaminoglycan

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Stem Cell Research & Therapy

ISSN: 1757-6512