Fig. 5

H89 blocks the 8-Br-cAMP-induced differentiation of WJ-MSCs into ESC-like cells. (A) Flow cytometric analysis of cells in the five groups using antibodies against vimentin and CD13. (B) Flow cytometric analysis of cells in the five groups using antibodies against cytokeratin and CD9. (a) no inhibition group; (b) p38 MAPK-block group; (c) ERK1/2-block group; (d) PKA-block group; (e) no differentiation group. Cells in Groups a–d treated with 8-Br-cAMP for 21 days