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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Single cell transcriptomics suggest that human adipocyte progenitor cells constitute a homogeneous cell population

Fig. 2

a Second-level clustering of ASCs. Left: cell–cell (upper) and gene–gene distance matrices (lower) of cells and genes ordered according to cluster membership determined by first-level clustering. Pearson correlation used as distance metric. Right: heatmap showing normalized expression of genes (rows) over all cells (columns) in the dataset. Cells and genes ordered as shown on the left. Upper panel shows total number of unique molecules per cell, while lower panel shows cluster membership. Apparent from both the heatmap and the cell–cell clustering, there were no apparent subpopulations of ASCs present in the data. b t-SNE visualization of ASCs based on gene modules selected in a. A minimum spanning tree through the data and the corresponding diameter path are shown. Cells colored according to position in pseudotime. c Rolling-wave plot showing the spline-smoothed expression patterns of significant pseudotime-dependent genes ordered according to pseudotime point of peak expression. Upper panel shows pseudotime position of ASCs colored according to patient ID. Lowest panel shows position of four pseudotime bins corresponding to the most prominent expression patterns. Example genes peaking in all four bins shown on the right. d Expression patterns of example genes introduced in C projected onto t-SNE visualization of ASCs (left) and SVF cells (right). e Position of pseudotime bins introduced in C projected onto t-SNE visualization of ASCs (left) and SVF cells (right)

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