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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Regenerative potential of primary adult human neural stem cells on micropatterned bio-implants boosts motor recovery

Fig. 4

a hNSC location and survival around neuro-implants 3 months after graft. hNSCs were identified by two specific human markers (red), hMTCO2 or hNCAM, in combination with a marker (green) of immature (Tuj-1) or mature (NeuN) neurons. Low magnification on the left and higher magnification, corresponding to the area in the white square, on the right. White arrows indicate blood vessels (Axiozoom images, scale bar: 100 μm). b hNSC detection 3 months after a graft of hNSCs alone. hNSCs identified in red by hMTCO2 and in green by Tuj-1. Low magnification on the left and higher magnification, corresponding to the area in the white square, on the right. White arrows indicate rare surviving hNSCs among a cluster of non-bright fluorescent cells corresponding certainly to non-alive cells (Axiozoom images, scale bar: 100 μm). V ventricle, L lesion, hNSC human neural stem cell, hMTCO2 human mitochondria marker (cytochrome c oxidase II), hNCAM human neural cell adhesion molecules, Tuj1 neuron-specific class III beta-tubulin, NeuN neuronal nuclear antigen

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