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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Regenerative potential of primary adult human neural stem cells on micropatterned bio-implants boosts motor recovery

Fig. 5

Immunofluorescence staining of hNSCs on neuro-implants 3 months after engraftment. a Typical distribution of hNSCs and their neuritic network on the PDMS scaffold stained with the neuronal markers (green) NeuN (top), SMI312 (middle) and MAP2 (bottom). Scale bars: 500 and 20 μm. b Detection of cell differentiation after 3 months on implants pre-seeded with hNSCs (hNSC Implants group) or on their own (Implants Alone group). Single or double staining was performed as indicated, with the human markers hMTCO2 or hNCAM (red) and markers for immature (nestin and Tuj1 in green and DCX in red) and mature (NeuN in green) neurons. Nuclei counterstained with DAPI. White arrowheads indicate marker co-localization and arrows indicate single marker expression. Axiozoom images, scale bar: 20μm. hNSC human neural stem cell, hMTCO2 human mitochondria marker (cytochrome c oxidase II), hNCAM human neural cell adhesion molecules, Tuj1 neuron-specific class III beta-tubulin, MAP2 microtubule associated protein 2, NeuN neuronal nuclear antigen, DCX doublecortin

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