Fig. 6

Immunofluorescence characterization of hNSC differentiation in reconstructed and host peri-lesional tissues. Double staining performed as indicated, with human markers (hMTCO2 or hNCAM) and markers for immature (nestin and Tuj1) and mature (MAP2 and NeuN) neurons. Representative double staining shown at higher magnification in the insets. Nuclei counterstained with DAPI. White arrows indicate blood vessels, white arrowheads indicate human fibres and asterisks localize typical double staining. Confocal images, scale bar: 20 μm. hMTCO2 human mitochondria marker (cytochrome c oxidase II), hNCAM human neural cell adhesion molecules, Tuj1 neuron-specific class III beta-tubulin, MAP2 microtubule associated protein 2, NeuN neuronal nuclear antigen