Fig. 7

Localization of migrant hNSCs grafted in the brain on implants, detected by immunofluorescence staining. Identification of the migration area on three axial slices (Paxinos and Watson atlas [32]) at Bregma coordinates: –3.60 mm, –4.60 mm and –5.82 mm. Lesion indicated by blue circle on slices of the whole brain, and red rectangles represent implants. hNSCs identified by the specific human markers (red, hMTCO2 and hNCAM), in combination with different markers of maturation (green, nestin, Tuj1 or NeuN). Names of structures indicated at the level where hNSCs were retrieved. AV antero-ventral thalamic nucleus, CA3 CA3 field of hippocampus, ChP choroid plexus, CPu caudate/putamen, M1 primary motor cortex, M2 secondary motor cortex, S1 primary somatosensory cortex, VPM ventral posteromedial thalamic nucleus, VPL ventral posterolateral thalamic nucleus, hMTCO2 human mitochondria marker (cytochrome c oxidase II), hNCAM human neural cell adhesion molecules, NeuN neuronal nuclear antigen