Skip to main content
Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Regenerative potential of primary adult human neural stem cells on micropatterned bio-implants boosts motor recovery

Fig. 8

Assessment of astrocyte and inflammation reaction near cystic cavity and implantation site. a Typical appearance of fluorescent immunostaining for GFAP, the astroglial marker (green) and ED1, a cellular marker specific to activated microglia, monocytes and macrophages (red) on Axiozoom images. White squares indicate higher magnification area of insets. Scale bar: 500 μm. b Immunostaining quantification of the astroglial signal: data expressed as the surface of GFAP-positive signal/mm2 of tissue analysed in each group (n = 5 per group). c Number of ED1 immunoreactive cells/mm2 of tissue analysed (n = 5 per group). Data represent median ± interquartile range. Values analysed with the Kruskal–Wallis test followed by corrected Dunn’s test. *p < 0.05. ***p < 0.0001. d Immunofluorescence detection of host or human astrocytes in the tissue of hNSC Implants rats 3 months post graft. Astrocytes (GFAP) in green and hNSCs (hNCAM) in red. Representative double staining shown at higher magnification. Scale bar: 50 μm. L lesion, V ventricle, hNSC human neural stem cell, GFAP glial fibrillary acidic protein, hNCAM human neural cell adhesion molecules

Back to article page