Fig. 1

The expression pattern of Cxs in differentiation of WB cells. a Representative images of WB cells, hepatocytes (WB-Hep) and biliary epithelial cells (WB-BEC) differentiated from WB cells. b qRT-PCR analysis of hepatic and biliary markers in WB-Hep and WB-BEC. c Immunostaining of Cx32 and Cx43 in WB cells and WB-Hep. d Western blotting of hepatic markers in WB cells and WB-Hep; β-actin was used as an internal control. Scale bars:100 μm for A and 50 μm for C. Data represented as mean ± SEM. ^* p < 0.05, ^** p < 0.01