Isolation and prolonged expansion of oral mesenchymal stem cells under clinical-grade, GMP-compliant conditions differentially affects “stemness” properties

Table 1 Primers designed for real-time PCR and the respective amplicon sizes of the products

Gene symbol	Type of marker	Forward (5′–3′)	Reverse (5′–3′)	Amplicon size (bp)
BMP-2	Osteogenic	GGAACGGACATTCGGTCCTT	AGTCCGTCTAAGAAGCACGC	100
ALP	Osteogenic	CCGTGGCAACTCTATCTTTGG	CAGGCCCATTGCCATACAG	89
RUNX2	Osteogenic	CCACCGAGACCAACAGAGTC	TCACTGTGCTGAAGAGGCTG	118
BGLAP	Osteogenic	GACTGTGACGAGTTGGCTGA	AAGAGGAAAGAAGGGTGCCT	137
ACAN	Chondrogenic	CACCTCCCCAACAGATGCTT	GGTACTTGTTCCAGCCCTCC	107
SOX9	Chondrogenic	AGGAAGTCGGTGAAGAACGG	CGCCTTGAAGATGGCGTTG	84
PPAR-γ	Adipogenic	GACAACCTGCTACAAGCCCT	TTGGCAAACAGCTGTGAGGA	71
LPL	Adipogenic	CGAGCGCTCCATTCATCTCT	CCAGATTGTTGCAGCGGTTC	137
B2M	Housekeeping	TGTCTTTCAGCAAGGACTGGT	ACATGTCTCGATCCCACTTAAC	138
SDHA	Housekeeping	GCATGCCAGGGAAGACTACA	GCCAACGTCCACATAGGACA	127

ISSN: 1757-6512