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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: miR-200c suppresses endometriosis by targeting MALAT1 in vitro and in vivo

Fig. 4

miR-200c regulates EMT in HESCs by downregulating MALAT1. a Western blot analysis of ZEB1, ZEB2, N-cadherin, and E-cadherin expression after transfection of HESCs with the miR-200c mimic (50 nM), NC mimic, miR-200c inhibitor (100 nM), or NC inhibitor for 48 hours. b Western blot analysis of ZEB1, ZEB2, N-cadherin, and E-cadherin levels after transfection of HESCs with siMALAT1(50 nM) or siNC for 48 hours. c EdU assay to evaluate the proliferation of HESCs treated with the miR-200c inhibitor (100 nM), NC inhibitor, siMALAT1 (50 nM), or siNC for 48 hours (magnification, 200×). d Number of proliferating cells calculated, depicted in the bar chart. e Transwell assay to evaluate the migration of HESCs treated with the miR-200c inhibitor (100 nM), NC inhibitor, siMALAT1 (50 nM), or siNC for 48 hours (magnification, 200×). f Number of migrated cells calculated, depicted in the bar chart. g Western blot analysis of ZEB1, ZEB2, N-cadherin, and E-cadherin expression after transfection of HESCs with the miR-200c inhibitor (100 nM), NC inhibitor, siMALAT1 (50 nM), or siNC for 48 hours. All data shown as mean ± SD of three independent experiments (*P < 0.05 and **P < 0.01, Student’s t test). EdU 5-ethynyl-2′-deoxyuridine, MALAT1 metastasis-associated lung adenocarcinoma transcript 1, Mimic NC negative control miRNA mimic, Inhibitor NC negative control miRNA inhibitor, siRNA small interfering RNA, siRNA NC negative control siRNA

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