Skip to main content
Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: Poly(ethylene glycol)-modified silk fibroin membrane as a carrier for limbal epithelial stem cell transplantation in a rabbit LSCD model

Fig. 9

Corneal epithelial regeneration and repair after LESC/SF graft transplantation. a Rabbit corneas 4 months after transplantation (left panels, corneal epithelial cells marker CK12 staining; middle panels, enlarged pictures of the framed area; right panels, proposed LESC marker ABCB5 staining in the limbus). The four groups are as follows: (1) cornea from normal eyes (positive control), (2) cornea from LSCD model (no grafts, negative control), (3) PEG-modified SF membrane transplantation (SF grafts), and (4) LESCs cultured on PEG-modified SF membrane transplantation (LESC/SF grafts). Arrows point to the ABCB5+ LESCs in the limbus. b Macrophage antigen CD68 (located at endosome, lysosome, and cell membrane) staining showed inhibited inflammation in the corneas 4 months after LESC/SF graft transplantations. Arrows point to CD68+ macrophages in the corneas. c Corneal epithelial thickness in the four groups. d Corneal stromal thickness in the four groups. e Area percentages of CK12+ epithelium in total corneal epithelium in the four groups. Transplantation experiments were performed in three rabbits per group. Three consecutive cross-sections from three rabbits per group were analyzed. Data was shown as mean ± SD. One-way ANOVA: *P < 0.05; **P < 0.01; n.s. not significant. Scale bar, 50 μm. ABCB5 sub-family B, member 5, CK12 cytokeratin12, DAPI 4′,6-diamidino-2-phenylindole, LESC limbal epithelial stem cell, SF silk fibroin

Back to article page