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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Caffeic acid phenethyl ester promotes haematopoietic stem/progenitor cell homing and engraftment

Fig. 2

CAPE promoted HSPC homing to the BM niche. a Schematic representation of the homing experiments. Lethally irradiated CD45.2 mice were transplanted with 2 × 107 CD45.1+ BM MNCs and injected with 3.0 mg/kg CAPE or vehicle daily for 3 days. b Representative dot plots for murine BM LinSca-1+CD45.1+CD45.2 cells. c Percentage of CD45.1+CD45.2LinSca-1+ cells in BM was increased by CAPE (n = 6). d Total CFU number generated from 5 × 105 BM MNCs. BM MNCs were harvested for CFU assays 20 h after BMT (n = 5). e Number of different types of CFU (BFU-E, CFU-G/GM/M and CFU-GEMM) generated from 5 × 105 BM MNCs. f Homing efficiency of CFUs calculated by comparing the homed CFU number with the initially injected CFU number. Blue indicates vehicle-treated group, red indicates CAPE-treated group; experiments repeated three times. Data presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, CAPE vs vehicle. TBI Total body irradiation, BM bone marrow, BMT bone marrow transplantation, CAPE caffeic acid phenethyl ester, SSC side scatter, CFU-G granulocyte colony-forming units, CFU-M macrophage colony-forming units, CFU-GEMM granulocyte–erythroblast–macrophage–megakaryocyte colony-forming units, CFU-GM granulocyte–macrophage colony-forming units, MNC mononuclear cell, NS not significant

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