Fig. 5

CAPE upregulated the expression of HIF-1α, VEGF-A and SDF-1α. a Gene expression levels of HIF-1α, VEGF-A and SDF-1α detected using Q-PCR. BMECs were incubated with CAPE at different concentrations (0, 0.1, 1.0 μg/ml) for 24 h. All gene expression data normalized by the housekeeping gene, β-ACTIN. ***P < 0.001, CAPE 1.0 μg/ml vs CAPE 0 μg/ml, CAPE 0.1 μg/ml vs CAPE 0 μg/ml. b Protein expression levels of HIF-1α, VEGF-A and SDF-1α in murine BMECs. BMECs were incubated with CAPE at different concentrations (0, 0.1, 1.0 μg/ml) for 24 h. Total protein of these cells was isolated and 60 μg protein per sample was loaded for western blot analysis. c Expression of HIF-1α, VEGF-A and SDF-1α proteins in the BM niche of lethally irradiated mice 20 h after BMT by IHC. Lethally irradiated mice were treated with vehicle or 3.0 mg/kg CAPE for three doses. All tests were repeated three times. CAPE caffeic acid phenethyl ester, HIF-1α hypoxia inducible factor-1α, NS not significant, SDF-1α stromal cell-derived factor 1α, VEGF vascular endothelium growth factor