Fig. 6From: Thioredoxin mitigates radiation-induced hematopoietic stem cell injury in miceTXN reduced γH2AX and p38 expression after TBI. Lin– BMCs were isolated and culture with appropriate growth factor as described in the text. Lin– BMCs were irradiated with 3 Gy, and treated with and without thioredoxin (TXN) for 1 and 4 h. a Western blot analysis of p38, phospho-p38, γH2AX, and GAPDH protein expression. Quantitative densitometry of the protein expressions of b phospho-p38 and c γH2AX. d Primary fibroblasts were irradiated with 3 Gy with and without TXN for 1 h and stained with γH2AX foci (red) and DAPI (blue). Data are shown as the mean ± SD. *P < 0.05; **P < 0.01. n.s., no statistical difference. IR ionizing radiation, n.s. not significant, UT untreatedBack to article page