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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Calcium-containing scaffolds induce bone regeneration by regulating mesenchymal stem cell differentiation and migration

Fig. 4

CaSO4 modulates migration and AKT activation induced by BMP-2. a, b Wound healing assay evaluating the effects of CaSO4 3 mM and BMP-2 2nM alone or in combination on MSC migration after 24 hours of culture. Results shown as average of three different experiments with six replicates for each condition. A representative image displayed for each condition. Note that CaSO4 and BMP-2 produced a similar effect on MCS migration. c Wound healing assay to assess effects of LY294002 or Wortmannin (PI3K/AKT inhibitors at 10 μM and 500 nM respectively) on MSC chemotaxis. Migration was abrogated when MSCs were treated simultaneously with CaSO4 and such inhibitors. d MSCs were cultured with CaSO4 3 mM and BMP-2 2nM alone or in combination for 24 hours, and extracts analyzed by western blot assay and quantified relative to α-Tubulin. Differences considered significant at **p < 0.01, and ***p < 0.001. BMP bone morphogenetic protein

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