Fig. 5From: Substance P and patterned silk biomaterial stimulate periodontal ligament stem cells to form corneal stroma in a bioengineered three-dimensional modelStromal ECM components deposited on patterned silk membranes with substance P (SP). PDLSCs were seeded on patterned silk membranes and cultured in SP-supplemented keratocyte differentiation medium for 14 days. The expression of collagens and proteoglycans was evaluated by immunofluorescence staining. The right panels of the patterned + SP images show the merged picture of the left panels (ECM component staining) and the middle panels (DAPI staining). White arrows indicate the direction of the grooves on patterned silk membranes. The far right panels show the negative control (NTC) without primary antibodyBack to article page