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Table 1 Primers used for quantitative polymerase chain reaction

From: Substance P and patterned silk biomaterial stimulate periodontal ligament stem cells to form corneal stroma in a bioengineered three-dimensional model

Genes 5′–3′ Primers
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) Forward TGACGCTGGGGCTGGCATTG
Reverse GGCTGGTGGTCCAGGGGTCT
Scleraxis (SCX) Forward CGAGAACACCCAGCCCAAAC
Reverse CTCCGAATCGCAGTCTTTCTGTC
Mohawk (MKX) Forward GAAGGCAACTTTGTCTATCGCA
Reverse TGATCTCCTTCCAATACGTGTC
Collagen type I (COL I) Forward CGATGGATTCCAGTTCGAGTAT
Reverse CATCGACAGTGACGCTGTAGG
Collagen type XIV (COL XIV) Forward AAGGATTGCCCTCCGACTACAC
Reverse CTGATGCGTTCATTGCCTTCTC
Nuclear factor of activated T-cells 4 (NFATC4) Forward AAGGGTGAGACGGACATCG
Reverse CCGCCCATTGGAGACATAA
Biglycan (BGN) Forward GATGGCCTGAAGCTCAA
Reverse GGTTTGTTGAAGAGGCTG
EPH receptor A4 (EPHA4) Forward AGTGGGCTGTGACAATCTGGAATA
Reverse CATTTAGACGGAACTGAGGAGGGT