Fig. 3

Difference of adipogenesis between ATSCs and BMSCs in vitro. a–d Total RNA extracted from ATSCs and BMSCs when cells were subjected to AIM for 7 days. Relative expression levels of PPARγ, CEBPα, AP2, and LPL checked by qRT-PCR. β-actin as an internal control. Data expressed as mean ± SD (n = 3). *p < 0.05, compared with MSCs in OIM; #p < 0.05, compared with MSCs in α-MEM. e, f ATSCs and BMSCs cultured in AIM for 21 days, then cells fixed and stained with Oil Red O. AP2 adipocyte protein 2, ATSC adipose tissue-derived MSC, CEBPα CCAAT/enhancer-binding protein alpha, BMSC bone marrow-derived MSC, LPL lipoprotein lipase, MEM minimum essential medium, MSC mesenchymal stem cell, OIM osteogenic induction medium, PPARγ peroxisome proliferator-activated receptor gamma