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Table 3 Human gene primer sequences

From: Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells

Gene

Forward primer (5′–3′)

Reverse primer (5′–3′)

GAPDH

AGGTGCGTGTGAACGGATTTG

TGTAGACCATGTAGTTGAGGTCA

RUNX2

TCAACGATCTGAAGATTTGTGGG

GGGGAGGATTTGTGAAGACGG

BMP2

TTCGGCCTGAAACAGAGACC

CCTGAGTGCCTGCGATACAG

ALPL

ACTGGTACTCAGACAACGAGAT

ACGTCAATGTCCCTGATGTTATG

OCN

GGCGCTACCTGTATCAATGG

GTGGTCAGCCAACTGGTCA

OPN

GAAGTTTCGCAGACCTGACAT

GTATGCACCATTCAACTCCTCG

  1. GAPDH glyceraldehyde-3-phosphate dehydrogenase, RUNX2 runt-related transcription factor 2, BMP2 bone morphogenetic protein 2, ALPL alkaline phosphatase, OCN osteocalcin, OPN osteopontin
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Stem Cell Research & Therapy

ISSN: 1757-6512