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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats

Fig. 2

Vitamin C promotes rat bone marrow stromal cell survival in vitro. a Flow cytometry analysis of cell apoptosis on days 1, 3, and 7 after seeding. Upper row: BMSCs in normal culture medium; lower row: BMSC encapsulated in PF-127 hydrogel (20%, w/v). b Quantification of BMSC apoptosis flow cytometry analysis, with and without PF-127. c The morphology of rat bone marrow stromal cells cultured in PF-127 supplemented with indicated concentrations of vitamin C for 3 days and 7 days. Scale bar = 10 μm. d Cell count kit (CCK) assay showing the cell growth of BMSCs in PF-127 supplemented with 0 μM, 50 μM, and 100 μM vitamin C at day 3 and day 7. Two-way ANOVA, followed by Bonferroni post-hoc tests; **P < 0.01, ***P < 0.001. e ELISA results show the concentration of major growth factors and cytokines produced by rat bone marrow stromal cell at day 3 after seeding. Data are presented as mean ± SD. Student’s t test, n = 3; *P < 0.05, ***P < 0.001. EGF epidermal growth factor, HGF hepatocyte growth factor, IGF insulin-like growth factor, IL interleukin, TNF tumor necrosis factor

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