Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13287-017-0719-7/MediaObjects/13287_2017_719_Fig5_HTML.gif)
Effects of chitosan/hyaluronic acid (CS/HA) NP exposure on IPFP mesenchymal stem cell (MSC) and articular chondrocyte (AC) cocultures. a Effect of the weight ratio of CS to HA on the size and zeta potential of NPs. Insert: TEM micrograph of CS/HA NPs. b Fluorescent images of cells exposed to FITC-conjugated CS/HA NPs for 1 h. c Morphology, hematoxylin and eosin (H&E), and Alcian blue staining of monolayer (top) and pellet (bottom) culture of MSCs + ACs + NPs. d Immunofluorescence assay of monolayer (top) and pellet (bottom) culture of MSCs + ACs + NPs. Col collagen, NC negative control