Table 1 Primers used for RT-PCR and qRT-PCR analysis
Gene | Forward primer | Reverse primer |
|---|---|---|
GAPDH | TCCTCTGACTTCAACAGCGA | GGGTCTTACTCCTTGGAGGC |
CCNA2 | CAGCCAGACATCACTAACAGT | CCCACAAGCTGAAGTTTTCCT |
CCNB1 | GTTGGTGTCACTGCCATGTT | TGGCCAAAGTATGTTGCTCG |
CCND1 | GTCTGCGAGGAACAGAAGTG | GGATGGAGTTGTCGGTGTAG |
CCNE1 | GGAAGAGGAAGGCAAACGTG | GCAATAATCCGAGGCTTGCA |
OCT4 | GACAGGGGGAGGGGAGGAGCTAGG | CTTCCCTCCAACCAGTTGCCCCAAAC |
c-MYC | AGAAATGTCCTGAGCAATCACC | AAGGTTGTGAGGTTGCATTTGA |
REX1 | ACCAGCACACTAGGCAAACC | TTCTGTTCACACAGGCTCCA |
KLF4 | ATAGCCTAAATGATGGTGCTTGG | AACTTTGGCTTCCTTGTTTGG |
NANOG | TGCAAGAACTCTCCAACATCCT | ATTGCTATTCTTCGGCCAGTT |
SeV* | GGATCACTAGGTGATATCGAGC | ACCAGACAAGAGTTTAAGAGATATGTATC |
KOS-tg* | ATGCACCGCTACGACGTGAGCGC | ACCTTGACAATCCTGATGTGG |
Klf4-tg* | TTCCTGCATGCCAGAGGAGCCC | AATGTATCGAAGGTGCTCAA |
cMYC-tg* | TAACTGACTAGCAGGCTTGTCG | TCC ACATACAGTCCTGGATGATGATG |
SOX7 | TGAACGCCTTCATGGTTTG | AGCGCCTTCCACGACTTT |
HAND1 | CCAGCTACATCGCCTACCTG | CCGGTGCGTCCTTTAATCCT |
ACTA2 | GTGATCACCATCGGAAATGAA | TCATGATGCTGTTGTAGGTGGT |
MYL2 | TACGTTCGGGAAATGCTGAC | TTCTCCGTGGGTGATGATG |
NESTIN | CAGCGTTGGAACAGAGGTTGG | TGGCACAGGTGTCTCAAGGGTAG |
BMP4 | CCTGTTGTGTGCCCACTGAAC | ATCTCAGCGGCACCCACAT |